Y.H. He  , W.Q. Lu  , D.F. Li  , H.L. Zhang  , H.Q. Jiang  , 
    Effects of Soluble and Insoluble Non-starch Polysaccharides Isolated from Wheat Bran on Endogenous Amino Acid Loss at the Terminal Ileum of Growing Rats,
    Journal of Animal and Veterinary Advances,
    Volume 5,Issue 2,
    2006,
    Pages 143-149,
    ISSN 1680-5593,
    javaa.2006.143.149,
    (https://makhillpublications.co/view-article.php?doi=javaa.2006.143.149)
    Abstract: The objective of this study was to investigate the effects of soluble and insoluble non-starch polysaccharides isolated from wheat bran on endogenous amino acid flow at the terminal ileum of growing rats using  the  protein-free  method.  In  experiment  1,  20  Sprague-Dawley  rats  (200 ± 0.9 g),  were  fed  either  a protein-free, low fiber diet based on cornstarch, sucrose and soybean oil or a similar diet containing 50 g kg <SUP>1</SUP> of soluble non-starch polysaccharides added at the expense of cornstarch.  In experiment 2, 40 Sprague-Dawley rats (198 ± 0.8 g), were randomly allotted to either the protein-free, low fiber diet or similar diets containing either 50 g kg <SUP>1</SUP> insoluble non-starch polysaccharides, 50 g kg <SUP>1</SUP> soluble non-starch polysaccharides or a combination of 50 g kg <SUP>1</SUP> soluble and 50 g kg <SUP>1</SUP> insoluble non-starch polysaccharides added at the expense of cornstarch. Chromic oxide (6 g kg <SUP>1</SUP> diet) was included in all diets as an indigestible marker.  Both experiments lasted 8 d. The rats were killed on day 8 and the digesta contained in the final 20 cm of the ileum was obtained for assay. Compared  with  the  protein-free  basal diet, adding 50 g kg <SUP>1</SUP> soluble non-starch polysaccharides significantly (p&lt;0.01) increased the endogenous flow of amino acids and nitrogen at the terminal ileum (both experiment 1 and 2). Including 50 g kg <SUP>1</SUP> of insoluble non-starch polysaccharides also significantly increased (p&lt;0.05) the ileal flows of amino acids and nitrogen. The effects of the soluble non-starch polysaccharides were 22-85% greater than those of the insoluble non-starch polysaccharides.
    Keywords: Ileal endogenous amino acid loss;wheat;non-starch polysaccharides;rat