TY  - JOUR
T1  - Identification, CDNA Cloning and Characterization of
<I>Pax3</I> Gene in Chinese Domestic Goose
AU - Wang, Cui AU - Liu, Yi AU - Yang, Yunzhou AU - Wang, Huiying AU - Gong, Shaoming AU - Chen, Weihu AU - He, Daqian 
JO  - Journal of Molecular Genetics
VL  - 8
IS  - 1
SP  - 1
EP  - 8
PY  - 2016
DA  - 2001/08/19
SN  - 2070-4267
DO  - jmolgene.2016.1.8
UR  - https://makhillpublications.co/view-article.php?doi=jmolgene.2016.1.8
KW  - Goose
KW  -Pax3
KW  -gene cloning
KW  -bioinformatics analysis
KW  -alternatively spliced transcript
KW  -molecular
KW  -characterization
KW  -RT-PCR
KW  -gene expression
AB  - Paired box (Pax) protein 3 is a member of the Pax family of transcription factors, plays critical roles in
muscle development. Many studies showed that <I>Pax3</I> gene was a functional candidate gene for production
and meat quality. However, the sequence information of goose <I>Pax3</I> gene remains unknown. In this study, the
comparativegenomicstechnology was used to clone the cDNA sequence of <I>Pax3</I> gene from the breast muscle
tissue of Zhedong goose. The gene structures were analyzed by the bioinformatics software and the mRNA
expression profile of <I>Pax3</I> gene in different tissues was measured by the semi-quantitative RT-PCR. The goose
Pax3 full-length coding sequence consisted of 1327 bp and encoded 421 amino acids. Sequence analysis
displayed that a splicing variant of goose Pax3 absence of a glutamine residue was also identified. Both of the
Pax3-a (inclusion of a glutamine residue) and Pax3-b (exclusion of a glutamine residue) isoforms were predicted
contain three conserved domains (a paired domain, an octapeptide region and a homeodomain) and were highly
conserved (>90%) relative to the known Pax3 proteins from other species. Multiple sequence alignments and
phylogenetic analysis displayed that the deduced goose Pax3 proteins have a close genetic relationship and
evolutional distance with the Pax3 proteins in other avian species, especially in ducks. The semi-quantitative
RT-PCR results revealed Pax3 mRNA was highly expressed in the breast muscle tissue, followed by the lung,
leg muscle and brain, little or no expression was observed in heart, liver, spleen, kidney, muscular stomach,
intestine and sebum. These findings will help us understand the functions of the <I>Pax3</I> gene and the molecular
breeding in Chinese domestic goose.
ER  -