TY  - JOUR
T1  - Construction of a Vector Containing Coding Sequence of Lipid Transfer Proten-2 
  (<I>LTP2</I>) Gene from Rice
AU - Zahraie, Zohreh AU - Ehsanpour, Aliakbar AU - Karimian, Mohammad AU - Miroliaie, Mehran AU - Ghaedi, Kamran 
JO  - Journal of Molecular Genetics
VL  - 3
IS  - 1
SP  - 1
EP  - 4
PY  - 2011
DA  - 2001/08/19
SN  - 2070-4267
DO  - jmolgene.2011.1.4
UR  - https://makhillpublications.co/view-article.php?doi=jmolgene.2011.1.4
KW  - Iran
KW  -enzymes
KW  -pGEX-6P-2 vector
KW  -LTP
KW  -electrophoresis
KW  -colony PCR
AB  - Lipid Transfer Proteins (LTPs) are group of proteins with average molecular weight in plants that have potential applications especially in drug delivery systems. This research has been designed with the aim to construction of a vector containing coding sequence of Lipid Transfer Proten-2 (<I>LTP2</I>) gene from rice. There is no intron in rice <I>LTP2</I> gene. In here, pGEX-6P-2 vector was used. First was extracted rice genome. Then, <I>LTP2</I> gene was amplified with specific primers and PCR technique. In the next stage vector and <I>LTP2</I> gene was digested with BamHI and XhoI restriction endonuclease enzymes. Then, digested vector and digested <I>LTP2</I> gene were ligated in appropriate molar ratios.
ER  -