TY - JOUR T1 - Investigations on Seroprevalence of Bovine Brucellosis in Northeastern, Sudan AU - Mohamed Elsheikh, Hanan AU - Abbas Mohamed-Ahmed, Sawsan AU - Ibrahim Khojali, Maha AU - Omer Hassan, Salaheddin JO - Veterinary Research VL - 5 IS - 2 SP - 13 EP - 15 PY - 2012 DA - 2001/08/19 SN - 1993-5412 DO - vr.2012.13.15 UR - https://makhillpublications.co/view-article.php?doi=vr.2012.13.15 KW - Sudan KW -brucellosis KW -prevalence KW -Bovine KW -misdiagnosed KW -sensitive AB - A total of 377 serum samples were collected from un-vaccinated cattle brought for slaughter in Northeastern, Sudan to determine the seroprevalence of Bovine Brucellosis (BB) and to evaluate preliminarily the in-house Rose Bengal Plate Test (RBPT). The RBPT and Indirect Enzyme Linked Immunosorbant Assay (I-ELISA) were used to screen all serum samples. The RBPT was carried out in two approaches, the standard RBPT and modified RBPT (mRBPT). The seroprevalence of BB based on I-ELISA and RBPTs were respectively 15.4% (58/377) and 14.3% (54/377). A total of 6.9% (4/58) of true positive was misdiagnosed as negative by both RBPTs whereas 37% (20/54) of strong positive reaction (+3) by mRBPT was agglutinated weakly (+1) by RBPT. ER -