TY  - JOUR
T1  - Isolation and Characterization of Bacteria from Sub-Adult African Mud Catfish (<i>Clarias
gariepinus</i>) from Selected Fish Farms in Ogun State, Nigeria
AU - Awe, F.A. AU - Ezeri, G.N.O. AU - Bankole, M.O. AU - Akinyemi, A.A. AU - Whenu, O.O. AU - Clarke, E.O. AU - Adeboyejo, O.A. AU - Owodeinde, F.G. AU - Olarinmoye, O.M. AU - Hammed, A.M. AU - Olanloye, A.O. 
JO  - Journal of Fisheries International
VL  - 16
IS  - 1
SP  - 1
EP  - 8
PY  - 2021
DA  - 2001/08/19
SN  - 1817-3381
DO  - jfish.2021.1.8
UR  - https://makhillpublications.co/view-article.php?doi=jfish.2021.1.8
KW  - bacteria
KW  -characterization
KW  -Isolation
KW  -Clarias gariepinus
AB  - The need for proper identification of bacterial
organisms from catfish cannot be overemphasized, hence,
it provides update information on emerging and existing
organisms thereby enriching the gene bank on fish
disease. Catfish samples were collected from Elegbeji,
Sanni, Kunle, Johnson, Adewale and Awosanya fish
Farms and taken to the Microbiology laboratory, Federal
University of Agriculture, Abeokuta, Ogun State for
analyses and samples were collected from flesh, gills and
intestine were subjected to microbial examination for
colonial, Morphological characteristic, Biochemical tests
and Molecular tests. Bio Edit was used for importing and
mining nucleotides sequences into Gene Bank. The results
revealed the following bacterial organisms: fish farms
(1 and 2) <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>,
<i>Aeromonas veronii</i>, <i>Bacillus subtilis</i> were identified
from the skin and Gills while <i>Enterococcus feacium</i> was
also identified from the intestines. At farms (3 and 4), the
Major bacterial organisms identified from skin, gills and
Intestines of the fish were <i>Staphylococcus aureus</i>,
<i>Pseudomonas aeruginosa</i>, <i>Aeromonas veronii</i>, <i>Bacillus
subtilis</i> while farms (5 and 6) had <i>Enterococcus feacium</i>
and Streptococcus agalactiae. The BLAST result is a
confirmation of the bio-chemical test earlier carried out
with percentage similarity ranging from 78-98% and their
accession number, the bacterial organisms identified were
<i>Aeromonas veronii</i>, <i>Enterococcus feacium</i>, <i>Pseudomonas
aeruginosa</i>, <i>Bacteria subtilis</i>, <i>Staphylococcus aureus</i>,
<i>Streptococcus agalactiae</i>. The bacterial organisms were
present on the fish but not invasive and only become
dangerous if conditions necessary for disease
initiation are present such as susceptible host,
virulent pathogen and favourable environment are
present at the same time. Therefore, preventive medicine
is the solution for fish farm management but additional
bacterium <i>Aeromonas veronii</i> was detected and
need to be added into Nigeria Gene bank data for catfish.
ER  -