TY  - JOUR
T1  - Effect of <i>Momordica balsamina</i> on Insulin Resistant C2C12 Skeletal Muscle Cell lines
AU - Siphosethu Ngubane, Phikelelani AU - Khumalo, Bongiwe AU - Siboto, Angezwa AU - Sibiya, Ntethelelo AU - Khathi, Andile 
JO  - Agricultural Journal
VL  - 16
IS  - 5
SP  - 50
EP  - 56
PY  - 2021
DA  - 2001/08/19
SN  - 1816-9155
DO  - aj.2021.50.56
UR  - https://makhillpublications.co/view-article.php?doi=aj.2021.50.56
KW  - Momordica balsamina
KW  -insulin resistance
KW  -saturated fat (palmitic acid)
KW  -C2C12
KW  -glucometer
AB  - Studies have reported that <i>Momordica
balsamina</i> (MB) increases glucose uptake in skeletal
muscle cells. The effects of <i>M. balsamina</i> on insulin
resistant skeletal muscle cells however, are yet to be
established. The aim of the study therefore, is to
investigate the glucose lowering effects of <i>M. balsamina</i>
in the palmitic acid induced insulin resistant (C2C12)
skeletal muscle cell lines, <i>in-vitro</i>. Cell viability was
conducted in muscle cells to examine the cytotoxic effects
of <i>M. balsamina</i>. Cell viability of C2C12 muscle cell lines
was measured by means of 3-(4,5-Dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide (MTT) assay. Insulin
resistance was induced in skeletal muscle cell line
(C2C12) using palmitic acid (1 mM) administered in each
well. Cells were trypsinised and seeded into 24-well
plates at a seeding density of 1.8&times;104 cells/well and
incubated for 24 h to permit attachment and growth of
cells to semi-confluency. Media was replaced with the
media containing palmitic (500 uL) acid then these cells
were incubated for 4 h. After 4 h, new media was added.
Media glucose concentration was measured at 12, 24 and
48 h with One Touch select glucometer. The lipid
peroxidation marker, malanoaldehyde (MDA) was
measured and the total antioxidants capacity.
Furthermore, glycogen storage was measured using
glycogen assay. No toxic effects were demonstrated in all
3 doses <i>M. balsamina</i>. The administration of MB
significantly increased glucose uptake at 48 h of
incubation by comparison to palmitic acid exposed
control cells. Interestingly, the combination of MB
and insulin significantly increased glucose uptake
by comparison to MB alone at 48 h. Furthermore,
MB-treated cells had an increase glycogen storage
and reduced reactive oxygen production. These
findings may suggest that MB possesses potential
health benefit such as insulin sensitising and antihyperglycaemia
through improving glucose uptake
and attenuating oxidative stress in skeletal muscle.
ER  -