TY - JOUR
T1 - Genetic Variation of Wild and Hatchery Populations of Indian Major Carp, Rohu (Labeo rohita) in Bangladesh Using Allozyme Marker
AU - Sharke, Rajib AU - Pattadar, Shib Nath AU - Mollah, Fazlul Awal AU - Sarder, Rafiqul Islam AU - Al Faroque, Abdullah
JO - Agricultural Journal
VL - 9
IS - 2
SP - 113
EP - 119
PY - 2014
DA - 2001/08/19
SN - 1816-9155
DO - aj.2014.113.119
UR - https://makhillpublications.co/view-article.php?doi=aj.2014.113.119
KW - Labeo rohita
KW -genetic variation
KW -Allozyme electrophoresis
KW -hatchery population
KW -wild population
AB - The genetic variation of wild and hatchery populations of
rohu individuals was analyzed using allozyme marker. The fish samples were collected
from 3 main wilds (Halda, Jamuna and Padma) and 6 hatcheries of 3 origin (Brahmaputra
and Rahim fish hatchery, Mymensingh; Rupali and Ma-fatema fish hatchery, Jessore;
Bismillah and Rupali fish hatchery, Comilla) covering a wide geographical distribution
of this species. The 6 enzymes (LDH, MDH, PGM, AAT, ADH GPI and G3PDH) encoded
by 7 presumptive loci were screened and 4 (Mdh-1*, Gpi-1*, Gpi-2* and Pgm*)
were found to be polymorphic which were interpretable in muscle with starch
gel electrophoresis. The mean proportion of polymorphic loci, the mean proportion
of heterozygous loci per individual, the average observed Heterozygosity (Ho)
and expected Heterozygosity (He) were 42.85, 15.77, 0.125 and 0.184%,
respectively for wild populations which were higher than the hatchery populations
(33.33, 12.20, 0.075 and 0.094%, respectively). The highest gene flow value
(26.725) and lowest population differentiation (0.009) found in the Halda and
the Jamuna River populations indicate the close relationship among them Based
on Nei (1972)s UPGMA dendrogram,
9 populations of rohu were divided into 2 major clusters by the genetic distance
of D = 0.092. The cluster-1 consisted of 3 wild populations and the cluster-2
consisted of 6 hatchery populations. The present study revealed that the considerable
genetic variation is maintained among the wild and hatchery populations of rohu.
ER -