TY  - JOUR
T1  - The Effect of C-Jun on Leydig Cells Proliferation of Elemental Status in Rongchang Piglets
AU - Zheng, Xiaobo AU - Xiao, Rong AU - Li, Jiao AU - Zheng, Zhiwan AU - Qing, Lijuan AU - Wang, Libin AU - Yao, Yinzhu AU - Wu, Boqing AU - Xu, Huihao AU - Xu, Maosen AU - Li, Jing AU - Wu, Qun AU - Ge, Liang 
JO  - Agricultural Journal
VL  - 8
IS  - 5
SP  - 256
EP  - 261
PY  - 2013
DA  - 2001/08/19
SN  - 1816-9155
DO  - aj.2013.256.261
UR  - https://makhillpublications.co/view-article.php?doi=aj.2013.256.261
KW  - C-jun
KW  -piglet
KW  -leydig cells
KW  -proliferate
KW  -China
AB  - Leydig cells were isolated from the testis of 3 week old Rongchang piglets. Its activity, purity and function were qualified before being cultured <I>in vitro</I>. Then, they were respectively treated with 0.125, 0.25, 0.5, 1 and 2 &#956;mol L<SUP>-1</SUP> c-jun ASODNs and the expression of c-jun mRNA were detected by FQ-PCR. To study the effect of c-jun on proliferation of elemental status leydig cells in piglets, the leydig cells had been treatedwith c-jun ASODNs and the growth inhibition ratio of leydig cells were detected by MTT method and cell cycle phase distribution were examined by flow cytometer analysis. The FQ-PCR assays results indicated that c-jun mRNA expression decreased with the rise of c-jun ASODNs concentration. Only 0.125 &#956;mol L<SUP>-1</SUP> c-jun ASODNs could reduce the expression of <I>c-jun</I> gene significantly (p&lt;0.01). And the expression no longer went down when the concentration was up to 1 &#956;mol L<SUP>-1</SUP>. Through MTT analysis, it is found that c-junASODNs could inhibit the growth of leydig cells significantly (p&lt;0.01). There is a positive correlation between c-jun ASODNs concentration and growth inhibition ratio of leydig cells (r = 0.8966).
ER  -