TY  - JOUR
T1  - Scriptaid Improves Histone Acetylation and <I>in vitro</I> Development of Porcine Somatic Cell Nuclear Transfer Embryos
AU - Shi, Deshun AU - Wu, Zhengsan AU - Liu, Qingyou AU - Zhu, Peng AU - Liu, Shuai AU - Bian, Guihua AU - Yang, Sufang AU - Lu, Fenghua 
JO  - Agricultural Journal
VL  - 8
IS  - 4
SP  - 157
EP  - 164
PY  - 2013
DA  - 2001/08/19
SN  - 1816-9155
DO  - aj.2013.157.164
UR  - https://makhillpublications.co/view-article.php?doi=aj.2013.157.164
KW  - Development
KW  -histone acetylation
KW  -porcine
KW  -scriptaid
KW  -somatic cell nuclear transfer
AB  - Effects of Scriptaid (SCR), low toxicity Histone Deacetylase inhibitors (HDACi) on the histone acetylation and development of porcine Somatic Cell Nuclear Transfer (SCNT) embryos were examined in this study. Treatment of activated reconstructed SCNT embryos with 500 nM SCR for 12 h resulted in a significantly increase in the blastocyst development compared to untreated group (21.0% vs. 9.7%, p&lt;0.05). Further, SCR treatment resulted in SCNT embryos at the 2-cell, 4-cell and blastocyst stage with higher acetylation level of H3K18 in comparison with untreated group and similar to fertilized counterparts. Measurement of four selected developmentally important genes (<I>HDAC2</I>, <I>HAT1</I>, <I>OCT4</I> and <I>Phosphoglycerate kinase 1</I> (<I>PGK1</I>)) in blastocysts showed that treatment of SCNT embryos with SCR resulted in an increase expression in HAT1 and decrease expression in HDAC2 and PGK1. A similar expression profile of these genes was found in blastocysts developed from SCR treated SCNT embryos and <I>In Vitro </I>Fertilization (IVF) embryos. These results suggest that SCR treatment can improve the development competence of porcine SCNT embryos and result in a similar expression pattern of genes related to histone acetylation and acetylation level of histone compared to IVF embryos.
ER  -