TY  - JOUR
T1  - Developing Culture Media from Yam Species (<I>Dioscoreae rotundata)</I>: Technique 
  for Isolation of Tuber-Rot Fungi of Yam
AU - Nwawuisi, J.U. AU - Odoh, I.N. AU - Anaele, U.M. 
JO  - Agricultural Journal
VL  - 7
IS  - 4
SP  - 260
EP  - 263
PY  - 2012
DA  - 2001/08/19
SN  - 1816-9155
DO  - aj.2012.260.263
UR  - https://makhillpublications.co/view-article.php?doi=aj.2012.260.263
KW  - Yam
KW  -culture
KW  -media
KW  -rot-causing
KW  -fungi
KW  -Nigeria
AB  - In an experiment to develop yam (<I>Dioscorea rotundata</I>) based culture media for isolation of tuber-rot fungi of yam (<I>Dioscorea</I> sp.), culture media were prepared from three varieties of <I>Dioscorea rotundata</I>, namely Nwopoko, Obioturugo and Abi, for isolation of six causing rot fungi of yam with Potato Dextrose Agar (PDA) as standard culture medium. The six rot fungi were: <I>Botryodiplodia theobromae</I>, <I>Fusarium oxysporum</I>, <I>Penicillium sclerotigenum</I>, <I>Aspergillus niger</I>, <I>Rhizopus nodosus</I> and <I>Mucor circineloides</I>. The result of the study showed that the growth of the fungi on the yam-based culture media was equally as good as their growth on the Potato Dextrose Agar (PDA) used as standard medium, while some even gave better growth of the fungi with sporulation as high as 1.4x10<SUP>8 </SUP>and 1.6x10<SUP>8</SUP> obtained, respectively on Nwopoko and Obioturugo-based agar medium.
ER  -