TY  - JOUR
T1  - Inhibitory Properties of <I>Tinospora crispa </I>Extracts on TNF-&#945; Induced Inflammation on Human Umbilical Vein Endothelial Cells (HUVECS)
AU - Safwan Kamarazaman, Ihsan AU - , Khairul Kamilah Abdul Kadir AU - , Zamree Md Shah AU - , Mohd Shahidan Mohd Arshad AU - , Mohd Kamal Nik Hassan AU - , Khairunnuur Fairuz Azman AU - , Daryl Jesus Arapoc AU - , Abdah Md Akim AU - , Rasadah Mat Ali AU - , Zulkhairi Hj. Amom 
JO  - International Journal of Tropical Medicine
VL  - 7
IS  - 1
SP  - 24
EP  - 29
PY  - 2012
DA  - 2001/08/19
SN  - 1816-3319
DO  - ijtmed.2012.24.29
UR  - https://makhillpublications.co/view-article.php?doi=ijtmed.2012.24.29
KW  - TNF-a inflammation
KW  -HUVECs
KW  -Tinospora crispa
KW  -cardiovascular diseases
KW  -Malaysia
AB  - There were accumulating evidences that relate the occurrence of atherosclerosis and inflammation in the intima of arteries. Atherosclerosis itself has been known as chronic inflammatory disease. Many signaling molecules such as ICAM-1, VCAM-1, MCP-1, M-CSF and NO have been found in the atherosclerotic plague of the arteries in heart disease patients. This study aimed to investigate the effect of <I>Tinospora crispa</I> Aqueous Extract (TCAE) and Methanol Extract (TCME) on Tumor Necrosis Factor (TNF)-&#945; induced inflammation on Human Umbillical Vein Endothelial Cells (HUVECs) <I>in vitro</I>. HUVECs were cultured on 6 wells plate before been treated by TCAE and TCME at various concentrations (100, 200, 400 and 600 &#956;g mL<SUP>-1</SUP>). After 1 h of incubation, TNF-&#945; (10 ng mL<SUP>-1</SUP>) was exposed on HUVECs. HUVECs were harvested after 24 h and tested for ICAM-1, VCAM-1, M-CSF and NO using kit. Results of this study indicated that TCAE and TCME exert inhibitory effect on TNF-&#945; induced secretion of ICAM-1, VCAM-1 and M-CSF signaling molecule while NO secretion was increased. These results showed that <I>T. crispa </I>extracts has inhibitory effect <I>in vitro</I> on the level of inflammatory signaling molecules thus it may have a potential benefits on the development of nutraceuticals in the prevention of atherosclerosis-related cardiovascular diseases.
ER  -