TY - JOUR
T1 - In-House Rose Bengal Plate Agglutination Test (RBPT) for a Rapid Diagnosis of Brucellosis in Goats in Malaysia
AU - Shahaza, O. AU - Khairani-Bejo, S. AU - Zunita, Z. AU - Bahaman, A.R.
JO - International Journal of Tropical Medicine
VL - 4
IS - 3
SP - 116
EP - 118
PY - 2009
DA - 2001/08/19
SN - 1816-3319
DO - ijtmed.2009.116.118
UR - https://makhillpublications.co/view-article.php?doi=ijtmed.2009.116.118
KW - Goat
KW -brucellosis
KW -Rose Bengal Plate Test (RBPT)
KW -diagnosis
KW -CFT
AB - The Rose Bengal Plate Test (RBPT) antigens from Brucella melitensis local isolates (in-house RBPT) were prepared and compared with RBPT antigen for Brucellosis in sheep and goats prepared by Veterinary Laboratory Agency, UK. Eight hundred fifty-six sera samples, of which were collected from goats were examined with the RBPT and Complement Fixation Test (CFT). The RBPT and CFT results showed that the in-house RBPT antigen was superior to the commercial prepared RBPT antigen (VLA, UK). Out of 856 sera analyzed by in-house RBPT, commercial RBPT and CFT, 30.84, 26.40 and 31.65% were found to be Brucella positive, respectively. The sensitivity calculated for the in-house RBPT compared with CFT was 85.24% whilst that of commercial RBPT was 78.59%. Therefore, it was conclude that in-house RBPT antigens could be prepared and used for epidemiological surveillance of Caprine brucellosis in Malaysia.
ER -