TY  - JOUR
T1  - <I>In vivo</I> and <I>In vitro</I> Effect of Sulfamerazine on Hepatic Mixed Function Oxidase in Rats
AU - Dandge, P.B. AU - Govindwar, S.P. 
JO  - Research Journal of Pharmacology
VL  - 5
IS  - 5
SP  - 53
EP  - 58
PY  - 2011
DA  - 2001/08/19
SN  - 1815-9362
DO  - rjpharm.2011.53.58
UR  - https://makhillpublications.co/view-article.php?doi=rjpharm.2011.53.58
KW  - Aminopyrine N-demethylase
KW  -aniline hydroxylase
KW  -cytochrome P-450
KW  -mixed function oxidase
KW  -sodium
KW  -sulfamerazine
AB  - Sulfamerazine (SMR) administration (i.p., 3 days) of different doses to adult male rats showed significant decrease in electron transport components and drug metabolizing enzymes. In longer duration study, 100 mg SMR kg<SUP>-1</SUP> caused significant decreases in cytochrome P-450 and activities of aminopyrine N-demethylase and aniline hydroxylase. In inhibitory dose of 100 mg SMR kg<SUP>-1</SUP> was selected for dosing young male, old male and adult female rats. Sulfamerazine administration to young, old male and female rats resulted in a significant decrease in electron transport component and drug metabolizing activities at 100 mg SMR kg<SUP>-1</SUP> dose level. All other parameters were unchanged. Sulfamerazine resulted in uncompetitive type of inhibition (Ki = 3.0 mM) of aminopyrine N-demethylase <I>in vitro</I>. Sulfamerazine destructed the spectral and catalytic activity of cytochrome P-450. The studies suggest that SMR is a substrate of the mixed function oxidase system and inhibition is dependent on dosage, age and sex of the animals.
ER  -