TY - JOUR T1 - Optimization for the Extraction of Antioxidant Compounds from Justicia adhatoda Leaves Using Response Surface Methodology AU - Rony, Satyajit AU - Hossain, Md. AU - Akhter, Shammi AU - Sohrab, Md. AU - Afroz, Farhana AU - Begum, Mst. JO - Research Journal of Pharmacology VL - 14 IS - 4 SP - 49 EP - 57 PY - 2020 DA - 2001/08/19 SN - 1815-9362 DO - rjp.2020.49.57 UR - https://makhillpublications.co/view-article.php?doi=rjp.2020.49.57 KW - Central composite design KW - experimental designs KW - quadratic polynomial equation KW - analysis of variance KW - antioxidant extract KW - radical scavenging activity AB -

In this study, the response surface methodology (RSM) constructed on a central composite design (CRD), was applied to control optimization for the extraction  of  antioxidant  compounds  from Justicia  adhatoda leaves. Two extraction parameters were assessed at high and low levels (13  experimental  designs): Methanol (30-80%) and the extraction time (4-6 days). Using RSM, a quadratic polynomial equation was used by multiple regression analysis to predict the optimization of the extraction process. Analysis of variance (ANOVA) was applied and the significant effect of the factors and their interactions were tested at a 95% confidence interval. The antioxidant extract (AE) yield was significantly influenced by solvent composition and time. The maximum AE was obtained at methanol (55%) and time (6.41 days) 6.48 g/100 g at run 13. The minimum radical scavenging activity (IC50) values were obtained at (methanol/45% water and extraction time 3.59 days) 1.28 μg mL‾1. It was found that the antioxidant extract yields and radical scavenging  activity  (IC50) of Justicia adhatoda leaves could be optimized at 3.10 g/100 g and 17.10 μg mL‾1 at the optimum condition at a time of 6.06 days and solvent concentration of 69.24%. The results of the antioxidant properties of Justicia adhatoda leaves by using response surface methodology (RSM) confirm this technique's usefulness.

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