TY  - JOUR
T1  - Development of Shrimp Holder Device for Artificial Insemination of Banana Shrimp <em>Penaeus merguiensis</em> (De Man, 1888)
AU - Memon, A.J. AU - Hidayati, N. AU - Talpur, A.D. AU - Khan, M.I. AU - Fariddudin, M.O. AU - Safiah, J. AU - Ikhwanuddin, M. AU - Abol-Munafi, A.B. 
JO  - Research Journal of Applied Sciences
VL  - 7
IS  - 1
SP  - 10
EP  - 16
PY  - 2012
DA  - 2001/08/19
SN  - 1815-932x
DO  - rjasci.2012.10.16
UR  - https://makhillpublications.co/view-article.php?doi=rjasci.2012.10.16
KW  - Banana shrimp
KW  -Penaeus merguiensis
KW  -molting
KW  -artificial insemination
KW  -SHDAI
KW  -spermatophore
KW  -fertilization
KW  -hatching
AB  - In aquaculture industry, spermatophore transfer technique such as artificial insemination in particular
with reference to banana shrimp <em>P. merguiensis</em> is challenging. The aim of this study is to examine a novel
technique for artificial insemination in <em>P. merguiensis</em> using SHDAI (Shrimp Holder Device for Artificial
Insemination). In order to transfer the spermatophore properly into thelycum, an appropriate shrimp holder with
continuous aeration system has been developed. During the process of manual spermatophore transfer
(artificial insemination) also, a protective device to keep the female <em>P. merguiensis</em> shrimps alive and under
minimum stress is necessary. The artificial insemination process carried out using SHDAI showed no signs of
stress and/or mortality of the broodstock. During the experiments, female shrimps were 100% alive and active.
Altogether, 78 female shrimps were tested of which 63 successfully accepted the spermatophore by using
SHDAI. The accepted spermatophore percentage was significantly higher and achieved as 80.76%. Accepted
spermatophore mass in the frozen, control and mean of both were recorded as 48.36&plusmn;10.45, 45.0&plusmn;15.28 and
46.68&plusmn;2.38% which indicated no significant differences (p<0.05) However, frozen sperm at -196&deg;C LN
up to 90 days was 64.1&plusmn;4.3% which indicated no significant differences between the SHDAI and control
(p<0.05). Whereas frozen sperm at 196&deg;C up to 90 days in LN was 62.5&plusmn;2.9% there was no significant difference
between the SHDAI and control (p<0.05). In the present study, overall quality of sperm in terms of the
fertilization rate and hatching rate were almost similar between inseminated (using SHDAI) and control (natural
mating) broodstock.
ER  -