TY - JOUR T1 - Prevalence of Streptococcus pneumoniae Serotypes (Nasopharyngeal Colonization) in Children in North Jordan: Genotypic and Phenotypic Characteristics AU - Masaadeh, Hani A. AU - Hayajneh, Wail A. AU - Al Azzam, Isra`a A. AU - Alkhatib, Ahed J. JO - Research Journal of Biological Sciences VL - 11 IS - 1 SP - 23 EP - 33 PY - 2016 DA - 2001/08/19 SN - 1815-8846 DO - rjbsci.2016.23.33 UR - https://makhillpublications.co/view-article.php?doi=rjbsci.2016.23.33 KW - Streptococcus pneumoniae KW -serotype KW -nasopharyngeal colonization KW -children KW -genotype KW -phenotype AB - Nasopharyngeal swabs were collected from children <5 years who attend King Abdullah Teaching Hospital and the emergency room at Princess Rahma Teaching hospital in North Jordan. S. pneumoniae identification was confirmed using colonial morphology, susceptibility to optochin and bile solubility. Sequential multiplex PCR test was used to determine capsular serotypes of S. pneumoniae isolates. 37.8% of children were carriers to capsulated S. pneumoniae. The most prevalent S. pneumoniae serotypes were 6A/B (13.2%), 23F (7%), ST14 (6%), 9V (4.4%), 11A (3.2%), 19F (3%), ST3 (1.8%), ST4 (1.8%), 12F (1.6%), 35B (1.4%), 19A (0.8%), 7F (0.6%). This type of colonization was not statistically related to acute illness. These moderately high colonization rates are expected to help future research to determine streptococcal strains causing invasive disease and subsequently help in making decision regarding the appropriateness of using new conjugate pneumococcal vaccines. ER -