TY  - JOUR
T1  - Influence of Physical and Chemical Parameters on Protease Production by <I>Bacillus megaterium</I> IBRL MS 8.2 in Submerged Fermentation
AU - Lim, S.H. AU - Darah, I. AU - Choo, M.L. 
JO  - Research Journal of Biological Sciences
VL  - 8
IS  - 2
SP  - 54
EP  - 61
PY  - 2013
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2013.54.61
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2013.54.61
KW  - Bacillus megaterium
KW  -submerged fermentation
KW  -proteases
KW  -enzyme production
KW  -Malaysia
AB  - Proteases are a group of enzymes that catalyze the hydrolysis of protein and among the various proteases, bacterial proteases are the most significant when compared to animal and fungal proteases. In this study, a local bacterial isolate <I>Bacillus megaterium</I> IBRL MS 8.2 was found to produce the highest protease production of 155.38 U mL<SUP>-1</SUP> when cultivating it in the improved medium which consisted of initial medium pH of 7.5, inoculum size of 7.5% (3x10<SUP>7</SUP> cells mL<SUP>-1</SUP>), agitation speed of 150 rpm, incubation temperature of 30&deg;C, 0.5% of sucrose and 1.0% of beef extract. The highest protease production was achieved on the 30 h of cultivation. On the other hand, the highest cell growth of 2.33 g L<SUP>-1</SUP> was achieved on 18 h of cultivation time. The data obtained suggest that enzyme production by <I>B. megaterium</I> IBRL MS 8.2 was not growth dependant.
ER  -