TY  - JOUR
T1  - DNA Barcoding of <I>Lates calcarifer </I>(Bloch, 1970)
AU - Prasannakuma, C. AU - Lyla, P.S. AU - Jalal, K.C.A. AU - Khan, S. Ajmal AU - John, Akbar 
JO  - Research Journal of Biological Sciences
VL  - 5
IS  - 6
SP  - 414
EP  - 419
PY  - 2010
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2010.414.419
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2010.414.419
KW  - Malaysia
KW  -codon position
KW  -India
KW  -Latidae
KW  -COI
KW  -Cytochrome oxidase subunit I
AB  - An effort to assess the utility of 650 bp Cytochrome oxidase subunit I (DNA barcode) gene in delineating the members closely resembled marine fin fishes belonging to the family Latidae was made. To address the issue, all 40 barcode sequences of fishes belonging to Latidae family available at NCBI (National Centre for Biotechnological Information) along with the barcode data generated from commonly available fish Asian sea bass (<I>Lates calcarifer</I>) from Parangipettai coastal waters was used. The average GC content of selected three fishes belongs to Latidae was found to be 48.87%. <I>Lates niloticus </I>from Tanzanian waters showed less GC content (47.70%) whereas <I>L. calcarifer</I> from South China sea showed high GC content (49.61%) among the <I>Latidae</I> species studied. Compare to Australian, Indian and Singapore (48.1-48.89%) water <I>L. calcarifer</I>, Myanmar and South China species shows high average GC content (49.38-49.41%). The phylogenetic and genetic distance data showed that the maximum genetic distance is present in 3rd codon position in all the selected species. It is apparent from the genetic distance that the GD between <I>L. calcarifer</I> and <I>L. niloticus</I> was observed to be higher than the GD between <I>P</I>. <I>waigiensis</I> and <I>L. niloticus</I> in all the codon positions. The expected clustering of the species in separate internal branches was well observed in the constructed phylogram with high bootstraps. We conclude that COI sequencing (barcoding) in identifying the morphologically similar species of the family Latidae.
ER  -