TY  - JOUR
T1  - Effects of Subinhibitory Concentrations of Antibiotics and Antibodies on the Adherence of <I>Escherichia coli</I> to Human Uroepithelial Cells <I>In vitro</I>
AU - Jahanshahi, Maryam AU - Azad, Saeed AU - Aslanbeigi, Bahram AU - Rahbar, Mohammad 
JO  - Research Journal of Biological Sciences
VL  - 5
IS  - 4
SP  - 326
EP  - 329
PY  - 2010
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2010.326.329
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2010.326.329
KW  - Sub MIC
KW  -uropathogenic E. coli
KW  -uroepithelial cells
KW  -ampicillin
KW  -trimethoprim
KW  -sulphadiazine
KW  -pili-antibodies
AB  - Uropathogenic <I>Escherichia coli</I> is an important cause of urinary tract infection. Adherence to uroepithelial cells is a first step for colonization of bacteria. The aim of this study was to determine effects of sub Minimum Inhibitory Concentrations (MICs) of antibiotics and antibodies on the adherence of this organism at<I> in vitro</I> condition. Seven strains of <I>E. coli</I> isolated from patients with acute pyelonephritis were subject of the study. MICs of trimethoprim, suphamethoxazole, sulphadiazine and ampicillin for these strains were determined and effect of these antibiotics at the &frac12; and &frac14; MIC on the adhesion of <I>E. coli</I> to human urinary tract epithelial cells were studied. Trimethoprim, sulphamethoxazole and sulphadiazine at &frac12; and &frac14; of the MICs decreased the adherence of four out of five <I>E. coli</I> strains tested whereas combinations of the compounds did not potentiate the effect. Ampicillin caused a similar effect. Specific pili-antibodies as well as gamma globulin and milk inhibited the adherence but did not work synergistically with ampicillin. In conclusion, present preliminary investigation gives further support to the theory that the effect of sub inhibitory concentration of antibiotics on the growth of bacteria may prevent their attachment to urinary epithelial cells.
ER  -