TY  - JOUR
T1  - Application of RT-PCR to Detect Treated and Untreated <I>Staphylococcus aureus </I>Genes with Marine Algae
AU - AL-Haj, Nagi A. AU - Masha, Nurmas I. AU - Shamsudin, Mariana N. AU - Mohamad, Habsah AU - Vairappan, Charles S. AU - Sekawi, Zamberi 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 8
SP  - 952
EP  - 959
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.952.959
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.952.959
KW  - Reverse transcription-polymerase chain reaction
KW  -Staphylococcus aureus
KW  -marine algae
KW  -antimicrobial activities
KW  -extraction
KW  -sequencing
KW  - Malaysia
KW  - Malaysia
AB  - Methacillin Resistant <I>Staphylococcus aureus</I> (MRSA), Extended Spectrum Beta Lactamase (ESBL) organisms and Multiple Drug Resistant Organism (MDRO). Therefore, this study was designed to explore an alternative antibacterial product derived from seaweed extracts, <I>Gracilaria changii</I> and <I>Euchema denticulatum</I>, through the study of DNA and RNA encoding genes of interest in MRSA and non-MRSA. The target of this study is to amplification of several untreated and treated <I>S. aureus </I>and<I> E. coli </I>genes that are potentially involved in the antibacterial activities through RT-PCR assay. <I>G. changii</I> and <I>E. denticulatum</I> extracts showed inhibitory activity against <I>S. aureus</I>, several genes in this pathogen were chosen to study the effect of both seaweed extracts on the genes through PCR and RT-PCR analysis. However, the predicted inhibitory mechanism of both seaweeds extracts on mecA gene was not fully elucidated in the study. The investigation could scientifically proof the natural products to be potentially potent antibacterial agents.
ER  -