TY  - JOUR
T1  - Expression of Bovine Leukemia Virus Tax Protein in Bacterial Cell
AU - , Hassan Momtaz 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 5
SP  - 542
EP  - 546
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.542.546
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.542.546
KW  - pET-32(a) vector
KW  -protein expression
KW  -immunoblotting
KW  -SDS-PAGE
KW  -E. coli
AB  - In order to cloning of the coding region of Tax gene of Bovine Leukosis Virus (BLV), PCR product of the open reading frame of the gene from BLV-FLK cell line and the buffy coat of infected cow were amplified by  PCR.  A  927  bp  PCR  product of the Tax gene with XhoI, BamHI restriction sites were subcloned of pCR 4-TOPO and digested by the mentioned endonucleases. Digested insert cloned in to pET-32(a) and transfected in <I>E.coli</I> cells. For the expression of Tax protein, the pET-32(a) recombinant vector was transformed and then induced in BL21 (DE3) strain of <I>E.coli</I> competent cells using IPTG, the presence of Tax expressed protein was shown in immunoblotting and SDS-PAGE system. With considering the significant prevalence of infection with BLV in Iran and the need for controlling the infection or disease through vaccination, the application of Tax recombinant protein for vaccine production is of great goals of this study in near future.
ER  -