TY  - JOUR
T1  - Genetic and Pathogenic Diversity of <I>Xanthomonas albilineans</I> (Ashby) Dowson, in Mexico
AU - , Manuel Huerta-Lara AU - , Reyna Isabel Rojas-Martinez AU - , Juliana Bautista-Calles AU - , Delfino Reyes-Lopez AU - , Marcelino Becerril-Herrera AU - , Omar Romero-Arenas AU - , Omar Franco-Mora AU - , Daniel Jimenez-Garcia AU - , Agustin Aragon-Garcia AU - , Alfredo Simon-Baez AU - , Dagoberto Guillen-Sanchez 
JO  - Research Journal of Biological Sciences
VL  - 4
IS  - 3
SP  - 312
EP  - 319
PY  - 2009
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2009.312.319
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2009.312.319
KW  - Genetic markers
KW  -diversity
KW  -leaf scald
KW  -sugarcane
KW  -Xanthomonas albilineans
KW  -resistance
AB  - The researching of genetic and pathogenic diversity of <I>Xanthomonas albilineans</I> (Xa) support directly the legal control and the genetic improvement of sugarcane (<I>Saccharum officinarum</I> L.). This researching was carried out with the objective to analyze genetic and pathogenic variability of the bacteria Xa from Mexico, applying isolated strain of syntomathic sugarcane from Veracruz (XaVer1 y XaVer2) and Chiapas (XaChis1 y XaChis2). The identification of Xa was carried out through PCR. The genetic variability was done using RFLP with the enzymes <I>Alu</I>I, <I>Kpn</I>I, <I>Tsp</I>5091, <I>Hpa</I>II y <I>Hha</I>I. The pathogenic variability was determined in the variety Mex 64-1487 in the greenhouse using a complete randomized design with 5 replications. The plants were inoculated with 1 mL, of 1.2×±0<SUP>8</SUP> CFU mL <SUP>1</SUP>, 6 days old bacterial suspensions or distilled water. The sequence of PCR amplified segment DNA of XaVer2 had a homology of 98% with an isolation of Xa coming from Brazil (Access: AF209751). The genetic markers got with <I>Alu</I>I, <I>Tsp</I>5091 and <I>Hpa</I>II allowed to different the Veracruz and Chiapas states, both in origin and in variability. The more pathogenic strains were XaVer2 and XaChis1 (p 0.05), both of them coming from susceptible varieties.
ER  -