TY  - JOUR
T1  - Optimization of<I> Brucella abortus</I> S99 Lipopolysaccharide Extraction by Phenol and Butanol Methods
AU - , Ali Sharifat Salmani AU - , Seyed Davar Siadat AU - , Dariush Norouzian AU - , Hojat Ahmadi AU - , Mehdi Nejati AU - , Bahman Tabaraie AU - , Seyed Mohammad Ataybi AU - , Mehrangize Zngeneh AU - , Ashraf Mohabati Mobarez AU - , Reza Shapour AU - , Mohammad Hassan Hedayati AU - , Mehdi Abbasi AU - , Maryam Karbasian 
JO  - Research Journal of Biological Sciences
VL  - 3
IS  - 6
SP  - 576
EP  - 580
PY  - 2008
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2008.576.580
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2008.576.580
KW  - Brucella abortus
KW  -lipopolysaccharide
KW  -optimization
KW  -extraction
KW  -phenol
KW  -butanol methods
AB  - <I>Brucella abortus</I> S99 Lipopolysaccharide (LPS) extracted and purified by phenol and butanol methods after submerged culture in the fermentor and then the optimization of these two methods carried-out by some modifications to increase the yield of extraction and LPS content and also to decrease the protein and nucleic acid content of extracted samples. Extracted samples chemically characterized by LPS and Ketodeoxyoctanate (KDO) content measurement and the evaluation of protein and nucleic acid contamination. LPS  and  KDO  content  of optimized phenol extracted sample have been more than other extracted  samples (146 EU mL <SUP>1</SUP> and 1.8%, respectively) and protein contamination of this sample has been less than the sample extracted by phenol (Westphal and Jann) method (2 versus 2.7%). Optimization of butanol method led to higher content of LPS and KDO in comparison with butanol (Morrison and Leive) method. Finally among four evaluated extraction methods (butanol, phenol, optimized butanol and optimized phenol), the optimized phenol extraction of <I>Brucella abortus</I> S99 lipopolysaccharide reported as the best method for industrial purposes (e.g. antigen  production  for diagnosis of brucellosis)  based  on  chemical  characterization  of  extracted  samples.
ER  -