TY  - JOUR
T1  - Endothelial Differentiation of Rat Mesenchymal Stem Cells
AU - , Sergey V. Tokalov AU - , Susanne Gruner AU - , Sebastian Schindler AU - , Nasreddin D. Abolmaali 
JO  - Research Journal of Biological Sciences
VL  - 2
IS  - 3
SP  - 307
EP  - 310
PY  - 2007
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2007.307.310
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2007.307.310
KW  - Bone marrow
KW  -MSC
KW  -DMEM
KW  -GM
AB  - Bone Marrow (BM) derived Mesenchymal Stem Cells (MSCs) are pluripotent cells which can replicate as undifferentiated cells, have potential to differentiate into endothelial, osteogenic, adipogenic and other lineages under determined culture conditions and are considered as a valuable source for the <I>in vivo</I> investigation of tumor angiogenesis, cell and gene based therapy. The aim of this study was to optimize <I>in vitro</I> expansion of MSCs derived Endothelial Progenitor Cells (EPCs) in a rat model. MSCs were isolated from bone marrow by a combination of gradient density centrifugation and adherence properties. <I>in vitro</I> cultures of MSCs were established using the 6 different commercially available Growth Media (GM) including DMEM, DMEM+VEGF, Endopan, MV2 and Panserin, and their potential to differentiate into osteogenic, adipogenic and endothelial cells was shown. By using Endopan MSCs exhibit the highest proliferation rate and the uppermost capacity to EPCs differentiation in comparison with other exploiting GM. Endopan is favorable medium for EPCs preparation. It may be very helpful in the different applications of EPCs in small-animal cell based diagnostics and therapeutic models.
ER  -