TY  - JOUR
T1  - Detection of Caprine-Specific Nucleic Acid Sequences in Goat Milk Using Polymerase Chain Reaction
AU - A. Osman, Asim AU - E. Aradaib, Imadeldin AU - A. Musa, Omer 
JO  - International Journal of Molecular Medicine and Advance Sciences
VL  - 9
IS  - 1
SP  - 5
EP  - 9
PY  - 2013
DA  - 2001/08/19
SN  - 1813-176x
DO  - ijmmas.2013.5.9
UR  - https://makhillpublications.co/view-article.php?doi=ijmmas.2013.5.9
KW  - rapid detection
KW  -DNA
KW  -Goat milk
KW  -PCR assay
KW  -cattle
AB  - This study was carried out to evaluate, PCR-based method for 
  detection of DNA in goat milk. It utilized primers targeting the mitochondrial 
  cytochrome b (<I>mtcyt-b</I>) gene which was used as a target DNA for PCR amplification. 
  For the specific identification of goat <I>mtcyt-b</I> gene, pair of primers 
  (GSL1, GSR2) were used which produced a 428 base pair (bp) PCR product from 
  milk samples as well as from peripheral blood. Amplification products were visualized 
  on ethidium bromide-stained agarose gels. Amplification products were not detected 
  when the PCR was applied to DNA from animal species including cattle, sheep, 
  swine, camel, deer, horse, donkey and human which indicates that the 2 pairs 
  of primers are specific for goat. In conclusion, DNA can be extracted from goat 
  milk and would be advantageous in the variety of application such as species 
  identification in milk and milk.
ER  -