TY - JOUR T1 - Effect of Various Protecting Compounds Added to the Growth Medium upon Survival of Lactobacillus sakei to Heating, Freezing, Freeze-drying and Storage in the Dried State AU - , Cristina Santos AU - , Joana Silva AU - , Paul A. Gibbs AU - , Paula Teixeira JO - Journal of Food Technology VL - 3 IS - 4 SP - 502 EP - 505 PY - 2005 DA - 2001/08/19 SN - 1684-8462 DO - jftech.2005.502.505 UR - https://makhillpublications.co/view-article.php?doi=jftech.2005.502.505 KW - AB - The aim of the present study was to investigate if the presence of sorbitol, myoinositol, xylose, mannose and Tween 80 in the growth medium increased the survival of L. sakei during heating, freezing, freeze drying and storage of freeze dried cells. Survival during freezing was enhanced by ca. 8% when glycerol was present in the growth medium. Viability during freeze-drying and storage in the dried state was improved by the presence of xylose in the growth medium. In addition to xylose, the presence of mannose and myoinositol protected cells during storage but not during drying. Cells grown in the presence myoinositol presented the highest D value at 55?C, 1.4 folder higher than control cells. In comparison with cells grown in MRS, total amino acids concentrations were the same order of magnitude for cells grown in the presence of mannose and myoinositol but were lower for cells grown in the presence of the other compounds. ER -