TY  - JOUR
T1  - Surveillance of Parasitic Diseases of Zoonotic Importance in Fishermen,
Some Fish and Shellfish Species
AU - Fadel, Hanaa Mohamed AU - El-Lamie, M.M Maather AU - Sallam, Nahla Hamed 
JO  - Journal of Animal and Veterinary Advances
VL  - 18
IS  - 6
SP  - 175
EP  - 186
PY  - 2019
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2019.175.186
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2019.175.186
KW  - Fishbone zoonotic trematodes
KW  -metacercarial infection
KW  -Cryptosporidium
KW  -fish
KW  -shellfish
KW  -fishermen
KW  -experimental infection
KW  -sequencing
AB  - To study the epidemiologic status of zoonotic parasitic diseases in some fish and shellfish species
and fishermen, a total of 120 freshly caught fish; 100 cat fish (<i>Clarias gariepinus</i>) collected from Nile
tributaries in Ismailia city, 20 keeled mullet (<i>Liza Carinata</i>), 400 alive marine shellfish; 100 shrimps (<i>Penaeus
japonicus</i>), 100 gastropods (<i>Thais carnifera</i>) and 200 bivalves (Paphia andulata) in addition to 15 fecal
samples were collected from fishermen at the shores of lake Temsah in Ismailia city, Egypt during (fall) 2017
and (Spring) 2018. The parasitological study revealed that the eggs of Fishborne Zoonotic Trematodes (FZTs)
and <i>Cryptosporidium</i> spp. oocysts were detected in 26.7 and 20% of the examined fishermen stool samples,
respectively. The prevalence of Encysted Metacercarial infection (EMC) of FZTs and cryptosporidiosis among
the examined fin fish and shellfish species were (74 and 24%) in cat fish, (5 and 30%) in keeled mullet, (50 and
28 %) in shrimp, (20 and 30%) in gastropod and (60 and 20%) in bivalve. The main clinical picture of EMC
affected fishes was slimness, haemorrhages all over the body with respiratory distress. Affected <i>Penaeus
japonicus</i> were aggregated at the aerator sites while <i>Thais carnifera</i> and <i>Paphia andulata</i> showed no specific
clinical picture. Cryptosporidiosis affected fishes showed emaciation, ascites and haemorrages along the
stomach and intestine. Experimental infection in pigeons and rats, revealed <i>Brachylaema migrans</i> from pigeons
fed on <i>Penaeus japonicus</i> and (<i>Mesostephanus appendiculatus, Mesostephanus burmanicus, Procervum
calderoni, Stictodora tanayensis, Apophallus donicus, Pygidiopsis genata, Maritrema orensense,
Sphaeridiotrema globules, Prohemistomum vivax and Haplorchis pumilio</i>) from albino rats fed on <i>Clarias
gariepinus</i>. Some FZTs could not be identified by conventional morphologic typing, so, PCR targeting ITS2
(internal transcribed spacer) region was performed followed by bidirectional sequencing which revealed the
following worms: <i>Braunia cordiformis, Cyathocotyle prussica and Holostephanus dubinini</i>.
ER  -