TY  - JOUR
T1  - Possible Reproduction of Silky via. Transferred Primordial Germ Cells (PGCs)
AU - Yoshida, Tatsuyuki AU - Furuta, Hiroki 
JO  - Journal of Animal and Veterinary Advances
VL  - 16
IS  - 8
SP  - 75
EP  - 77
PY  - 2017
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2017.75.77
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2017.75.77
KW  - Chicken
KW  -silkie
KW  -germ line chimera
KW  -primordial germ cell
KW  -right side
KW  -settled
AB  - This experiment aimed to develop a procedure for transferring Primordial Germ Cells (PGCs) from silkie
chicken embryos into white leghorn embryos. We also examined the migratory abilities of these transferred
silkie chicken PGCs. Fertilized eggs of silky were incubated until Stages 11-17 of embryonic development. Blood
was collected from the blood vessels of silkie chicken embryos. PGCs were then isolated from blood cells,
labeled with PKH26 and injected into the blood vessels of white leghorn embryos at Stages 11-15. Silkie chicken
PGCs were found to migrate to the germinal ridges of white leghorn embryos at Stages 36. The number of PGCs
that settled on the left side germinal ridges was higher as compared with that settled on the right side.
ER  -