TY  - JOUR
T1  - Multiple Factors Contribute to Persistent Porcine Epidemic Diarrhea Infection 
  in the Field: An Investigation on Porcine Epidemic Diarrhea Repeated Outbreaks 
  in the Same Herd
AU - Dekun, Chen AU - Song, Changxu AU - Leng, Zhangming AU - Sun, Ruiqin 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 6
SP  - 410
EP  - 415
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.410.415
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.410.415
KW  - PED persistent infection
KW  -PEDV transmission
KW  -PEDV shedding time
KW  -semen
KW  -aerosol
AB  - In the recent years, severe reoccurrence of Porcine Epidemic Diarrhea (PED) has been recorded in China. In the present study, specific PED related symptoms were observed repeatedly up to six times in the same herds. To investigate the possible factors involved in the PEDV persistent infections and repeat outbreaks. Feces, semen and air samples were collected and examined by RT-PCR and quantified by real-time RT-PCR. The results showed that all of the spike gene sequences of samples at different time were highly homologous. PEDV was shed in fecal excretions at high concentration (up to 1x10<SUP>6.85</SUP> copies mL<SUP>-1</SUP>) and the shedding time lasted for at least 56 days under field condition. Semen collected from boars without PED clinical phenomena and air samples were positive for PEDV RNA as well.
ER  -