TY  - JOUR
T1  - Molecular Characterization and Expression Pattern of a Novel Flavonol Synthase 
  gene
AU - Leifeng, Zhao AU - Fuzhao, Nian AU - Yonghui, Zhang AU - Xiangyun, Su AU - Juqiu, Zou 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 9
SP  - 937
EP  - 941
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.937.941
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.937.941
KW  - Tobacco
KW  -gene
KW  -flavonol synthase
KW  -expression pattern
KW  -encode
AB  - Flavonols have significant health benefits for animals and humans. Flavonol synthase has been characterized to be required for flavonol biosynthesis. The complete coding sequence of tobacco Flavonol synthase gene was amplified by RT-PCR. The complete coding sequence of tobacco Flavonol synthase gene was 951 bp which encodes a protein of 316 amino acids. Sequence analysis revealed that the flavonol synthase of tobacco shares high homology with the flavonol synthase of <I>Lycopersicon esculentum</I> (69%), castor bean (64%), cucumber (58%) and chickpea (57%). Phylogenetic tree analysis revealed that the tobacco Flavonol synthase gene has a closer genetic relationship with that of <I>Lycopersicon esculentum</I>. Expression profile was studied and the results indicated that tobacco Flavonol synthase gene was highly expressed in in leaf and flower. These results established the primary foundation of utilization of tobacco flavonols in the future.
ER  -