TY  - JOUR
T1  - Influence the Content of Steroid Hormones and Expression of Steroidogenic Enzymes with DHEA-Treated in TM-3 Cells
AU - Ma, Haitian AU - Shen, Xuehuai AU - Chen, Di AU - Kang, Jian AU - Han, Jing AU - Liu, Guanxing AU - Zou, Sixiang 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 7
SP  - 831
EP  - 838
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.831.838
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.831.838
KW  - DHEA
KW  -steroid hormones
KW  -steroidogenic enzymes
KW  -TM-3 cell
KW  -cortisol
AB  - The adrenals of humans and primates could secrete large amounts 
  of Dehydroepiandrosterone (DHEA) and its sulphate ester (DHEA-S) in the circulation 
  which act as precursors of active steroid hormones in a long series of peripheral 
  target intracrine tissues. The marked decline of serum DHEA and DHEA-S concentrations 
  with age in humans has been incriminated in the development of various pathologies. 
  Therefore, this study aims to provide detailed information on the effects of 
  DHEA on steroid hormones and its metabolites by Liquid Chromatography-tandem 
  Mass Spectrometry (LC-MS) in TM-3 cells, a Leydig cell line. The results showed 
  that the concentration of DHEA rapidly declined post-treatment while testosterone 
  and estradiol content increased over the same time-period in TM-3 cells following 
  treatment with 100 μM DHEA. Concomitantly, the concentration of progesterone 
  slightly increased and cortisol content decreased over the treatment period 
  in TM-3 cell. Furthermore, DHEA treatment produced a significant increased in 
  the 3β-Hydroxysteroid Dehydrogenase (3β-HSD) and 17β-Hydroxysteroid 
  Dehydrogenase (17β-HSD) protein expression following incubation with DHEA 
  for 24-48 h but the protein expression of aromatase decreased in the presence 
  of DHEA for 24-48 h. The results of present study indicate that DHEA could transformed 
  into steroid hormones by regulated expression of steroidogenic enzymes in TM-3 
  cells.
ER  -