TY  - JOUR
T1  - Establishment and Characterization of a Fibroblast Line from Inner Mongolia Cashmere Goats
AU - Cui, Peng AU - Hu, Pengfei AU - Li, Xiangchen AU - Guan, Weijun AU - Ma, Yuehui 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 7
SP  - 823
EP  - 830
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.823.830
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.823.830
KW  - Inner Mongolia Cashmere goats
KW  -fibroblast
KW  -cell line
KW  -biological characteristics
KW  -marginal tissues
AB  - A fibroblast line (named SCF36) from 33 Inner Mongolia Cashmere 
  goats ear marginal tissues was established successfully by means of using primary 
  explant technique and cell cryoconservation technology. This fibroblast line 
  contains 336 cryovials with 8x10<SUP>6</SUP> cells, respectively. Biological 
  characteristics of the fibroblast line showed that the cells cultured <I>in 
  vitro</I> were all morphologically typical fibroblast and the cell Population 
  Doubling Time (PDT) was approximately 2 days. The cell average viability before 
  freezing was 97.37&plusmn;2.25 and 91.43&plusmn;.22% after thawing. Chromosome 
  analysis showed that &gt;90% of the whole population were diploid. Isozyme analysis 
  of Lactic Dehydrogenase (LDH) and Malic Dehydrogenase (MDH) showed that the 
  SCF36 cells had no cross-contamination with other species and the genetic characteristics 
  of the cell line were stable <I>in vitro</I>. Tests for cell line contamination 
  with bacteria, fungi and mycoplasmas were also negative. The transfection efficiency 
  of three fluorescent proteins were relatively high, indicating that the exogenous 
  genes could be effectively expressed in the cells. The cell line met all criteria 
  from the American Type Culture Collection (ATCC). Not only has the germline 
  of this important sheep breed been preserved at the cell level but also valuable 
  material had been provided for genome, postgenome and somatic cloning research 
  and so on.
ER  -