TY  - JOUR
T1  - Identification for Different Expression Proteins in Different Virulent <I>Streptococcus suis</I> Serotype 7 Strains
AU - Cai, Xuehui AU - Wang, Shujie AU - Li, Liqin AU - Liu, Siguo AU - Liu, Yonggang AU - Qi, Yingfang AU - Wang, Gang AU - Jiang, Yongqiang 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 4
SP  - 533
EP  - 538
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.533.538
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.533.538
KW  - SS7
KW  -comparative proteome
KW  -secreted proteins
KW  -qRT-PCR
KW  -PCR
AB  - <I>Streptococcus suis </I>(<I>S. suis</I>) secreted numerous proteins are virulence-associated factors that play critical roles in infection. Studies of<I> S. suis</I> secreted proteins have been reported in <I>S. suis</I> Serotype 2 (SS2) strains and<I> S. suis</I> Serotype 9 (SS9) strains. However, data is still scarce. In this study, a comparative proteomics approach was used to distinguish the profiles of the secreted proteins in different virulent SS7 strains. Consequently, 13 individual proteins that were unique to either high pathogenic 07WC11 strain or low pathogenic SJZB408 strain in 2D gels were successfully identified. In addition, researchers attempted to compare the amount of expression 10 identified protein genes (<I>pft</I>, <I>rsp</I>, <I>cms</I>, <I>esp</I>, <I>hpk</I>, <I>pk</I>, <I>rpp</I>, <I>rrf</I>, <I>tef</I>, <I>tis</I>) by quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). Real-time RT-PCR results showed that pft, rsp, cms, esp, pk, rpp, rrf and tef mRNA expression amount in low pathogenic SS7 strain was lower than in high pathogenic SS7 strain which is accord with the results of 2D. Thus, researchers defined these eight proteins as different expression proteins between low pathogenic and high pathogenic SS7 strain.
ER  -