TY - JOUR T1 - Identification of Monozygotic Twins in Pigs Using Optimized Fluorescent Microsatellite Markers AU - Li, Ming-Zhou AU - Lou, Ping-Er AU - Liu, Ying-Kai AU - Wang, Tao AU - Li, Xue-Wei JO - Journal of Animal and Veterinary Advances VL - 12 IS - 2 SP - 225 EP - 228 PY - 2013 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2013.225.228 UR - https://makhillpublications.co/view-article.php?doi=javaa.2013.225.228 KW - Pig KW -monozygotic twins KW -paired fluorescent microsatellite marker KW -STR genotyping KW -cumulative exclusion rate AB - Monozygotic (MZ) twins having the same DNA information have been used for many biomedical studies. Pigs present high similarity with humans and have been used as an ideal mammalian model the use of MZ twins is even more valuable. In the study, researchers present a systematic method for the stepwise exclusion of inconsistently genotyped individuals in a large population by Short Tandem Repeat (STR) genotyping. Five pairs of microsatellite markers with different fluorescent dyes were selected and their reliability was tested. The results demonstrate that the cumulative exclusion rate of all markers was 99.67% in 1,201 pigs; two pairs of MZ twins were initially identified. Researchers have established an effective, easy and cheap way to identify MZ twin pigs. ER -