TY  - JOUR
T1  - Acute Phase Protein Profile and Clinico-Pathological Changes in Mice Associated with the Infection of <I>Pasteurella multocida</I> Type B and 
  the Bacterial Lipopolysaccharide and Outer Membrane Protein Imminogens
AU - Jesse Abdullah, Faez Firdaus AU - Zakaria, Zunita AU - Abdullah, Rasedee AU - Saad, Mohd Zamri AU - Osman, Abdinasir Yusuf AU - Adamu, Lawan AU - Saharee, Abdul Aziz 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 2
SP  - 186
EP  - 193
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.186.193
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.186.193
KW  - bacterial lipopolysaccharide
KW  -Pasteurella multocida type B
KW  -outer membrane protein immunogens
KW  -Acute phase protein
KW  -infection
AB  - Haemorrhagic Septicaemia (HS) is a killer disease of cattle 
  and buffalo of economic importance in Asia and Africa. There is insufficient 
  information on the responses of Balb c mice as animal model in respect of immunogens 
  and Acute Phase Proteins (APP) profiles. Therefore, the present study aims to 
  evaluate the acute phase protein profiles in mice associated with the infection 
  of <I>Pasteurella multocida</I> type B and the bacterial lipopolysaccharide 
  and outer membrane protein immunogens. Two hundred healthy Balb/c mice of 8-10 
  weeks old were used in this study. They were divided into four equal groups 
  of 50 mice each. Mice of group 1 were inoculated intra-peritoneal with 1.0 mL 
  sterile Phosphate Buffered Saline (PBS) pH 7, group 2 were inoculated with 1.0 
  mL of 10<SUP>9</SUP> colony forming unit (cfu) of <I>P. multocida</I> B: 2. 
  Mice of groups 3 and 4 were inoculated intra-peritoneal with 1.0 mL of LPS and 
  1.0 mL of OMP, respectively. Acute phase proteins analysis were done using two 
  sites Enzyme Linked Immunoassay (ELISA) highly sensitive test kits. The data 
  was analyzed using SPSS. Haptoglobin concentration increased significantly in 
  group 3 and 4 (p&lt;0.05) following inoculation with immunogens compared to 
  control group. Mice in group 3 and 4 showed significantly (p&lt;0.000) 3 times 
  higher concentrations of SAA and significantly (p&lt;0.037) 1.3 times increased 
  concentrations of SAA, respectively compared to the control group. There was 
  no significant changes in the concentrations of fibrinogen in group 2 (p = 0.177), 
  group 3 (p = 0.088) and group 4 (p = 0.359). C-reactive protein in groups 2 
  and 3 showed significantly (p&lt;0.05) higher levels than the control group. 
  Albumin showed significant increase (p&lt;0.05) in group 2 compared to the control 
  group. There were significant changes in the concentrations of acute phase proteins 
  and clinical responses post inoculation with immunogens indicating adverse pro-inflammatory 
  reactions in mice in the present study.
ER  -