TY - JOUR
T1 - Molecular Characterization and Expression Pattern of a Novel Nicotinamide Adenine Dinucleotide Transporter Gene
AU - Leifeng, Zhao AU - Fuzhao, Nian AU - Yonghui, Zhang AU - Dingqi, Luo AU - Jianhua, Xia AU - Sheng, Jiang
JO - Journal of Animal and Veterinary Advances
VL - 12
IS - 15
SP - 1271
EP - 1275
PY - 2013
DA - 2001/08/19
SN - 1680-5593
DO - javaa.2013.1271.1275
UR - https://makhillpublications.co/view-article.php?doi=javaa.2013.1271.1275
KW - Tobacco
KW -nicotinamide adenine dinucleotide transporter 1
KW -expression pattern
KW -protein
KW -wine
AB - Nicotinamide adenine dinucleotide is a drug which is important
to the health of animals and humans. Nicotinamide adenine dinucleotide transporter
1 gene have been characterized to be required for nicotinamide adenine dinucleotide
bioactivety. In present experiment, the complete mRNA sequence of tobacco nicotinamide
adenine dinucleotide transporter 1 gene was amplified using the rapid amplification
of cDNA Ends Methods. The full-length tobacco nicotinamide adenine dinucleotide
transporter 1 gene mRNA was 1,588 bp containing an 948 bp open reading
frame which encodes a protein of 315 amino acids. BLAST analysis revealed that
tobacco nicotinamide adenine dinucleotide transporter 1 protein shares high
homology with the nicotinamide adenine dinucleotide transporter 1 of Lycopersicon
esculentum (93%), wine grape (86%), soybean (81%), chickpea (79%) and foxtail
millet (78%). Results also showed that tobacco nicotinamide adenine dinucleotide
transporter 1 gene has a closer genetic relationship with the nicotinamide adenine
dinucleotide transporter 1 gene of Lycopersicon esculentum. Prediction
of transmembrane helices showed that tobacco nicotinamide adenine dinucleotide
transporter 1 might be a transmembrane protein. The expression profile was studied
and the results indicated that tobacco nicotinamide adenine dinucleotide transporter
1 gene was highly expressed in leaf. These results established the primary foundation
of using tobacco nicotinamide adenine dinucleotide as drug for animals and humans
in the future.
ER -