TY  - JOUR
T1  - Detection of the Major Macrolide Resistance Genes in <I>Streptococcus suis</I> Serotype 2 Isolates in Hebei Province China
AU - , Zengjun Ma AU - Rui, Ping AU - , PeiGuo Li AU - , QinYe Song AU - , CaiRan Yang AU - , Ping Shen AU - , SuMin Pan AU - , Yufang Guo 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 6
SP  - 781
EP  - 784
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.781.784
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.781.784
KW  - Pigs
KW  -streptococcus
KW  -macrolides antibiotics
KW  -drug-resistance genes
KW  -GenBank
KW  -China
AB  - To investigate the distribution of ermB, ermA and mefA related to the erythromycin resistance gene, 32 drug-resistant <I>Streptococcus suis</I> type 2 isolates from different areas in Hebei province were studied to detect the <I>ermB</I>, <I>ermA</I> and <I>mefA</I> genes by PCR amplification. The results showed that among the drug-resistant 32 strains, 100% (32/32) amplified <I>ermB</I> gene, 21.88% (7/32) isolates contained <I>ermA</I> gene and none of these strains was positive for <I>mefA</I> gene. The researchers concluded that erythromycin resistance mechanism of pig Streptococcus isolates is mainly mediated by <I>ermB</I> gene. Nucleotide sequences comparison showed that the <I>ermB</I> gene of 20 strains and the nucleotide sequences in the GenBank had the sequence similarity of 95-100%. Compared with the reference sequence of AJ972604.1, Ser to Asn mutation at position 100 and Arg to His mutation at position 118 of ermB was mainly detected in the 20 strains. The researchers concluded that the <I>ermB</I> gene is relatively stable.
ER  -