TY  - JOUR
T1  - Characterization of MicroRNAs in <I>Paragonimus westermani</I> by Solexa Deep Sequencing and Bioinformatics Analysis
AU - Chen, J.X. AU - Ai, L. AU - Chen, M.X. AU - Chen, S.H. AU - Zhang, Y.N. AU - Li, H. AU - Cai, Y.C. AU - Lu, Y. AU - Tian, L.G. AU - Zhou, X.N. 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 18
SP  - 3469
EP  - 3473
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3469.3473
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3469.3473
KW  - Paragonimus westermani
KW  -microRNAs (miRNAs)
KW  -Solexa deep sequencing
KW  -novel
KW  -China
AB  - MicroRNAs (miRNAs) are small non-coding RNA which generate from large hairpin precursors and play important role on post-transcriptional regulators of target genes. Although, many individual miRNAs have recently been extensively studied, there has been very little research on miRNA transcriptomes in trematode. By the method of using high throughput Solexa sequencing technology, 11.02 million clean reads have been obtained of <I>Paragonimus westermani</I>. Among the clean reads, 1.14 million ones (10.37%) were perfectly mapped onto the <I>S. japonicum</I> genome which included 20,322 (0.69%) unique sequences. A number of 9,492,613 (86.17%) reads had no match with public databases and marked as un-annotated RNAs. Nucleotide bias analysis found that the known miRNAs showed high bias and the guanine was the dominant nucleotide, particularly at the 2nd and 23rd positions which were almost at the beginning and end of conserved miRNAs. Three novel miRNA corresponding to 18 precursors were predicted. To the knowledge, this is the first report of miRNA profiles in <I>P. westermani</I> which will contribute to better understanding of the complex biology of this zoonotic parasite. The reported data of <I>P. westermani</I> miRNAs should provide valuable references for miRNA studies of closed related zoonotic trematode.
ER  -