TY  - JOUR
T1  - Isolation and the Analysis of 16S rDNA Sequence of Swine<I> Actinobacillus 
  pleuropneumonia</I>
AU - Lin, Zhu AU - Hongping, Wan AU - Zhicai, Zuo AU - Tongchao, Fu AU - Hongbo, Dai AU - Lei, Chen AU - Zhiwen, Xu 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 18
SP  - 3276
EP  - 3280
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3276.3280
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3276.3280
KW  - Actinobacillus pleuropneumonia
KW  -pathogen isolate
KW  -16S rDNA
KW  -sensitive test
KW  -mouse
AB  - A highly infectious and fatal respiratory tract disease occurred in a swine farm in Sichuan province. To explore the reason of the disease, samples of the liver and lung tissue, acquired sterilely in a typically sickened pig were intended for pathogenic detection. The suspicious strain, named after SCAB-1 was diagnosed which depended on cultural characteristics, morphologic observation and Nicotinamide Adenine Dinucleotide (NAD) experiment. Its pathogenicity was diagnosed by the virulence test on mice. And the strain was exactly identified by the analysis of 16S rDNA sequence with its resistance inspected by sensitive test. According to cultural characteristics, morphology, NAD experiment and the analysis of 16S rDNA sequence, it revealed that the study triumphantly isolated a strain of <I>Actinobacillus pleuropneumonia</I> which could lead a mouse to death, after infected 16 h and depend on NAD. The homology of the 16S rDNA sequence between SCAB-1 and <I>Actinobacillus pleuropneumonia </I>is highly 99.9%. SCAB-1, <I>Actinobacillus pleuropneumonia</I> is susceptible to ceftriaxone, cefotaxime, cefuroxime, norfloxacin, SMZco; moderately sensitive to cefazolin, cefoperazone and resistance to cephaloridine IV, vancomycin and gentamycin.
ER  -