TY  - JOUR
T1  - Inflammatory Response of Peripheral Blood Mononuclear Cells Post Intramammary 
  Challenge with <I>Staphylococcus aureus</I> in Dairy Cows
AU - Xu, Shangzhong AU - Liu, Li AU - Yuan, Zhengrong AU - Chen, Cui AU - Zhang, Limin AU - Chen, Xiaojie AU - Liu, Xidong AU - Gao, Xue AU - Li, Junya AU - Gao, Huijiang AU - Zhang, Lupei 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 14
SP  - 2588
EP  - 2593
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2588.2593
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2588.2593
KW  - Dairy cow
KW  -mastitis
KW  -S. aureus
KW  -gene expression
KW  -PBMCs
KW  -China
AB  - To study the inflammatory response of Peripheral Blood Mononuclear Cells (PBMCs) to actue phases of <I>Staphylococcus aureus </I> (<I>S. aureus</I>) mastitis in dairy cows using gene expression data, the mRNA expression levels of six genes (Iinterleukin (IL)-8, Differentiation (CD) 14, Macrophage Colony Stimulating Factor Receptor (M-CSFR), Toll-Like Receptor 2 (TLR2), Chemokine Receptor 2 (CCR2) and CX<SUB>3</SUB>C Chemokine Receptor 1 (CX<SUB>3</SUB>CR1) in Peripheral Blood Mononuclear Cells (PBMCs) in infected cows (n = 3) with<I> S. aureus</I> relative to uninfected controls (n = 3) were measured at 0 day before inoculation and 1-3 days post inoculation (dpi) by the real-time quantitative PCR. Results indicated the expression of<I> IL-8</I>, <I>CD14</I> and <I>CCR2</I> gene were exhibited significantly (p&lt;0.05) up-regulated within 2 dpi while there was no significant difference in the expression of <I>M-CSFR</I>, <I>TLR2</I> and <I>CX<SUB>3</SUB>CR1</I> genes at 1-3 dpi. Additionally, concentrations of plasma lipopolysaccharide Binding Protein (LBP) and Somatic Cell Counts (SCCs) were evaluated. Together these data reveal that the upregulation of gene expression is probably related to the recruitment and activation of PBMCs.
ER  -