TY  - JOUR
T1  - Contribution of Insertion Within acrR to acrAB Expression and Ciprofloxacin Resistance 
  in a Veterinary <I>Escherichia coli</I> Isolate
AU - Jiang, Hong-Xia AU - Ren, Yan-Na AU - Li, Jian AU - Zhang, Xiao-Hua AU - Zeng, Zhen-Ling AU - Zhen, Pan-Pan AU - Zeng, Dong-Ping AU - Liu, Ya-Hong 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 14
SP  - 2436
EP  - 2439
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2436.2439
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2436.2439
KW  - Mutation
KW  - acrR
KW  -expression
KW  -E.coli
KW  -antibiotics
KW  -fragment
KW  -China
AB  - Mutation in acrR would result in enhanced expression of acrAB, increasing fluoroquinolone resistance and partially contributing to the increase in resistance levels to several unrelated antibiotics. An insertion of 777 bp fragment within <I>acrR</I> gene was detected in a clinical <I>E</I>. <I>coli</I> isolates from a diseased duck. In order to determine whether the insertion of acrR was responsible for the elevated expression of acrAB, complementation with wild-type acrR and expression levels of efflux pump and regulator genes by qPCR were carried out. Complementation led to increased sensitivity to ciprofloxacin and to ethidium bromide and decreased expression of acrA and acrB and increased expression of global regulators marA and SoxR compared with that of the parental strains suggesting that the insertion of acrR playing important role in increased acrAB expression and ciprofloxacin resistance.
ER  -