TY  - JOUR
T1  - Molecular Cloning, Sequence Characterization and Tissue Transcription Profile of a Novel Gene <I>PQBP1</I> from Chinese Banna Mini-Pig Inbred Line (BMI)
AU - Huo, Jinlong AU - Wang, Pei AU - Wei, Hongjiang AU - Zeng, Yangzhi 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 10
SP  - 1674
EP  - 1680
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1674.1680
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1674.1680
KW  - Banna Mini-pig Inbred line (BMI)
KW  -pig
KW  -PQBP1
KW  -tissue expression
KW  -bioinformatics analysis
KW  -China
AB  - The complete coding sequence of Banna Mini-pig Inbred line (<I>BMI</I>) gene PQBP1 was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to accession number JF750401. Sequence analysis revealed that the BMI PQBP1 encodes a protein of 265 amino acids that has high homology with the Polyglutamine Binding Protein1 (PQBP1) of five other species-human (95%), monkey (95%), cattle (95%), mouse (88%) and rat (87%). The phylogenetic tree analysis revealed BMI PQBP1 has a closer genetic relationship with the cattle PQBP1 than with those of human, monkey, mouse and rat. Analysis by RT-PCR showed that <I>BMI PQBP1</I> gene was over-expressed in midbrain, ovary, spleen, lung, nerve fiber, skin and muscle, moderately expressed in diencephalon, kidney, heart, stomach, small intestine, large intestine and fat, weakly expressed in lymph node and cerebrum and almost not expressed in liver and pancreas. One microRNA target site was predicted in the CDS of BMI PQBP1 mRNA for further studying this gene in the future. These data serve as a foundation for further insight into this swine gene.
ER  -