TY  - JOUR
T1  - Inhibition of Foot and Mouth Disease Virus Replication <I>in vitro</I> and 
  <I>in vivo</I> by Dual Short Hairpin RNA-Mediated RNA Interference
AU - Hazi, Wureli AU - Hu, Shengwei AU - Ni, Wei AU - He, Zhirui AU - Meng, Ren AU - Chen, Chuangfu AU - Xu, Ningying 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 10
SP  - 1527
EP  - 1531
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1527.1531
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1527.1531
KW  - FMDV
KW  -RNA interference
KW  -dual shRNAs
KW  -plasmid
KW  -genome
KW  -serotypes
AB  - Foot and Mouth Disease Virus (FMDV) is one of the most important pathogen to the cattle industry often resulting in severe economic losses. Researchers have reported previously a therapeutic application of plasmid-based shRNA against FMDV but the high degree of sequence diversity between different FMDV serotypes may result in the appearance of escape mutants. In this study, a dual shRNA expression plasmid which can simultaneously express two different shRNA molecules was established and showed stronger inhibitory effects on virus replication than the mixture of two shRNAs. Moreover, the antiviral activity induced by the dual shRNA expression system was aslo evident on other FMDV serotypes. Therefore, the dual shRNA system targeting two conserved regions of virus genome provides a more powerful strategy for inhibiting FMDV replication in a cross-resistance manner and implicates a potential application in the treatment of high genetic variability of FMDV.
ER  -