TY  - JOUR
T1  - Effects of Sijunzi Decoction on Small Intestinal T Lymphocyte Subsets Differentiation in Reserpine Induced Spleen Deficiency Rats
AU - , Jianqin Xu AU - Zhang, Nuowei AU - , Shining Guo AU - , Huanrong Li AU - , Jiefeng Li AU - Xu, Xiaolong AU - , Changrong Wan AU - , Hong Zhao AU - Liu, Faqiang AU - , Junlan Zan AU - , Bin Wang 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 9
SP  - 1290
EP  - 1298
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1290.1298
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1290.1298
KW  - Sijunzi decoction
KW  -lymphocyte subsets
KW  -reserpine induced
KW  -spleen deficiency
KW  -small intestine
KW  -China
AB  - Sijunzi decoction (SJZ), composed of Dangshen (<I>Codonopsis pilosula</I> Nannf.), Baizhu (<I>Atractylodes macrocephala</I> Koidz.), Fuling (Tuckahoe) and Gancao (Radix glycyrrhiza) is well known as a classical Chinese traditional and herbal medicamentum aiming directly at spleen deficiency in Traditional Chinese Medicine (TCM). The present research is to evaluate the regulatory effect of SJZ on small intestinal T lymphocyte subsets differentiation in reserpine-induced spleen deficiency rats. Reserpine induced spleen deficiency rats were orally administrated with SJZ (0.2 mL/200 g, 0.6 mL/200 g and 1.0 mL/200 g, body weight) once a day for a period of 5 days. Clinical symptoms including body weight were observed every day, the small intestine damage and recovery by SJZ were detected by electron microscope scanning, small intestine lymphocytes were separated for T lymphocyte subsets detection by flow cytometry and T lymphocyte related cytokine expression was studied using quantitative real time PCR. Results showed that proper dose of SJZ can significantly promote the recovery of body weight and small intestine damage as well as regulate the differentiation of CD3<SUP>+</SUP>, CD3<SUP>+</SUP>CD4<SUP>+</SUP> T lymphocyte subsets and the expression of Type 1 cytokines (IL-2, IFN-&#947; and IL10). Besides, a significant CD4&#175;CD8&#175; T lymphocyte subset was found in reserpine treated and high dose SJZ administrated rats. Data from this study proved that SJZ possess a significant promoting effect on the amelioration of spleen deficiency, CD4<SUP>+</SUP> T lymphocyte and cytokines related with regulation of T lymphocyte differentiation would be key targets of SJZ on spleen deficiency.
ER  -