TY  - JOUR
T1  - Effects of Glucose on Differentiation and Fat Metabolism of Chicken Preadipocytes
AU - , Sun Chao AU - Renli, Qi AU - , Yan Jun AU - , Yang Haili AU - , Zhao Taotao AU - , Zhao Xia 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 8
SP  - 1223
EP  - 1229
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1223.1229
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1223.1229
KW  - Glucose
KW  -chicken
KW  -preadipocyte
KW  -fat metabolism
KW  -cell differentiation
KW  -fatty acid transporter
AB  - The present study was carried out to illustrate the effect of glucose on chicken (<I>Gallus gallus</I>) preadipocyte differentiation and fat metabolism. Adipocyte differentiation was initiated by maintaining confluent cells in a glucose-free medium supplemented with different concentrations of glucose. Upon exposure to high concentrations of glucose (25 mmol L<SUP>-1</SUP>), Peroxisome Proliferator-Activated Receptor &#947; (PPAR&#947;) and CCAAT/Enhancer-Binding Protein &#945; (C/EBP&#945;) as adipocyte differentiation markers were significantly increased compared with control cells. The morphology of the glucose-treated cells changed from fibroblast-like to polygonal and cells treated with moderate concentrations of glucose (15 mmol L<SUP>-1</SUP>) accumulated the most number of cytoplasmic lipid droplets as estimated by Oil red O staining. Compared with control cells varying concentrations of glucose affected the mRNA expression and protein levels for Fatty Acid Synthesis (FAS) and Adipose Triglyceride Lipase (ATGL), the master regulators of fat metabolism. Moreover, the mRNA expression levels and protein contents of fatty acid transporters in glucose-treated cells were higher than in untreated cells. These results indicated that glucose is essential material for chick adipocytes differentiation. Moreover, overhigh concentrations of glucose maybe stimulate lipolysis in the cells.
ER  -