TY - JOUR
T1 - Determination of Kappa Casein Gene Polymorphisms and Their Effects on
Milk Composition in Some Native Cattle Breeds of Turkey
AU - Gurses, Murad AU - Yuce, Huseyin
JO - Journal of Animal and Veterinary Advances
VL - 11
IS - 7
SP - 1023
EP - 1027
PY - 2012
DA - 2001/08/19
SN - 1680-5593
DO - javaa.2012.1023.1027
UR - https://makhillpublications.co/view-article.php?doi=javaa.2012.1023.1027
KW - kappa casein (CSN3)
KW -Native cattle breeds
KW -gene polymorphism
KW -milk composition
KW -enzymes
AB - Polymorphisms in exon IV of bovine kappa-casein gene (CSN3) determine eleven
allelic variants (A, B, C, E, F1, F2, G1, G2, H, I and J) for the gene. These
variants are effective on milk production traits. The aim of this study was to
detect kappa-casein polymorphisms in Anatolian Black (AB) and East Anatolian
Red (EAR) native cattle breeds of Turkey. For this purpose, blood and milk samples
were collected from Middle and East Anatolian region of Turkey. Milk samples were
analyzed for protein, fat, lactose, solids, solids-not-fat content and density
by using milk analyzer. After DNA was extracted from the blood samples, exon IV
of CSN3 gene was amplified by PCR. PCR products were digested with HindIII
and HaeIII enzymes to distinguish allelic variants A, B and E. Genotype frequencies
were determined as AA = 0.500, AB = 0.500 for Anatolian Black and AA = 0.567,
AB = 0.433 for East Anatolian Red. Allele frequencies were determined as A = 0.750,
B = 0.250 for Anatolian Black and A = 0.783, B = 0.217 for East Anatolian Red.
The effects of genotypes on milk protein and SNF contents were found significant
in EAR (p<0.05) and also found close to significant in AB (p<0.1). The association
between CSN3 genotypes and milk composition suggests that CSN3 polymorphism might
be a useful marker for genotypic selection such as marker assisted selection.
Selection of B allele for CSN3 polymorphism could provide considerable economic
advantage for increasing milk quality.
ER -