TY  - JOUR
T1  - Development and Validation of a Gas Chromatography-Mass Spectrometry Method for the Simultaneous Determination of Melamine and Cyromazine in Animal Feeds
AU - Zhang, Liying AU - Yang, Wenjun AU - Wang, Zongyi AU - Chen, Yiqiang AU - Shang, Binru 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 1
SP  - 73
EP  - 80
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.73.80
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.73.80
KW  - determination
KW  -cyromazine
KW  -gas chromatography-mass spectrometry
KW  -feeds
KW  -Melamine
AB  - A new method for simultaneous determination of melamine and cyromazine in animal feeds using Gas Chromatography-Mass Spectrometry (GC-MS) was developed and validated. Samples were extracted with trichloroacetic acid solution cleaned up by cation exchange solid-phase extraction cartridges and derivatized with N, O-bis (trimethylsilyl) trifluroacetamide containing 1% trimethylchlorosilane followed by GC separation and MS detection. The limits of quantification were 0.10 mg kg<SUP>-1</SUP> for both melamine and cyromazine. Recoveries from feeds spiked at levels between 0.1 and 50 mg kg<SUP>-1</SUP> ranged from 84.2-99.5% with Relative Standard Deviation (RSD) &lt;8% with the exception of a 10.2% RSD for 0.1 mg kg<SUP>-1</SUP> melamine. This validated method was successfully applied to commercial feed samples showing that it can be used as a routine tool for the surveillance and evaluation of the presence of melamine and cyromazine in animal feeds.
ER  -