TY - JOUR T1 - Probiotic Activities of Lab in Endometritis: Probiotic Activities of Three Lactic Acid Bacteria Strains Isolated from Healthy Bovine Cervix: In Vitro Adherence to Immortalized Endometrial Epithelial Cells and Antimicrobial Properties AU - Jin, Yaping AU - Li, Yi AU - Bai, Dongning AU - Wang, Aihua AU - Zhao, Xianjun AU - Qi, Xuefeng JO - Journal of Animal and Veterinary Advances VL - 10 IS - 4 SP - 484 EP - 488 PY - 2011 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2011.484.488 UR - https://makhillpublications.co/view-article.php?doi=javaa.2011.484.488 KW - endometritis-associated pathogen KW -antimicrobial property KW -adherence KW -lactic acid bacteria KW -Immortalized endometrial epithelial cell KW -China AB - The ability of three Lactic Acid Bacteria (LAB), i.e., Lactobacillus acidophilus, Enterococcus faecalis and Enterococcus cecorum that isolated from cervical mucus in health cattle to adhere to immortalized Endometrial Epithelial Cells (EEC) and to affect the adhesion of two main endometritis-associated pathogens, Escherichia coli and Staphylococcus aureus were investigated. All probiotic strains exhibited strong adhesion levels to the cell lines and the adhesion levels of L. acidophilus was significantly higher as compared to that of E. faecalis or E.cecorum (p<0.05). In coculture assays, a significant decrease in the number of adhering pathogens was observed, using either preincubation, postincubation or coincubation of the pathogens with these LAB. L.acidophilus exhibited strong antagonistic activity against E. coli (p<0.01), nevertheless, E. Faecalis and E. cecorum showed strong inhibitory effect on S. aureus (p<0.01). In conclusion, the ability of LAB tested to inhibit the adhesion of endometritis-associated pathogens is highly specific and depends on both the probiotic and pathogen strains. ER -