TY  - JOUR
T1  - Prevalence of Methicillin-Resistant <I>Staphylococcus aureus</I> in Raw Milk and Dairy Products in Sarab by Culture and PCR Techniques
AU - Mirzaei, H. AU - Tofighi, A. AU - Karimi Sarabi, H. AU - Farajli, Mahdi 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 23
SP  - 3107
EP  - 3111
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.3107.3111
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.3107.3111
KW  - Methicillin-resistant Staphylococcus aureus
KW  -raw milk
KW  -dairy products
KW  -PCR
KW  -Iran
AB  - Recently Methicillin Resistant <I>Staphylococcus aureus</I> (MRSA) was isolated from dairy products in different countries. The aim of this study is to determine the prevalence rate of methicillin-resistant <I>Staphylococcus aureus</I> in raw milk, pasteurized milk, ice cream and traditional butter in Sarab by culture and PCR techniques. To do so, 200 samples of raw milk, pasteurized milk, ice cream and traditional cheese were collected from different sellers across Sarab. They were first evaluated for contamination by coagulase positive <I>Staphylococcus aureus</I> through culturing method and then, the isolates were subjected to PCR method according to <I>nuc </I>gene in order to confirm <I>Staphylococcus aureus</I> and methicillin resistance gene (<I>mecA</I>). The cultured samples indicated that 22 (44%) of raw milk samples, 4 (8%) of pasteurized milk samples, 9 (18%) of traditional butter samples and 12 (24%) of traditional cheese samples were contaminated by coagulase positive <I>S. aureus</I>. According to PCR using <I>nuc</I> gene primer, 18 (82%) of raw milk isolates, 4 (100%) of pasteurized milk isolates, 9 (75%) of traditional cheese isolates and 7 (78%) of traditional butter isolates contained <I>nuc </I>gene. According to PCR using <I>MecA</I> gene primer, 2 (50%) of pasteurized milk isolates and 2 (22%) of traditional cheese isolates contained <I>MecA</I> gene. No <I>MecA</I> gene was detected in raw milk and traditional butter isolates. The results provided evidence that the presence of coagulase positive <I>Staphylococcus aureus</I> and MRSA have become remarkably widespread in dairy product samples. This calls for better control of the sources of food contamination as well as the spread of antimicrobial-resistance organisms.
ER  -