TY  - JOUR
T1  - Short Hairpin RNA Targeted to the Highly Conserved Regions of <I>L</I> and <I>3D</I> Gene Inhibits Replication of Foot-and-Mouth Disease Virus <I>in vitro</I>
AU - Chen, Jin-Ding AU - Li, Yin-Guang AU - Chen, Li-Jun AU - Zhao, Ming-Qiu AU - Xu, Yan-Fang AU - Jia, Jun-Tao AU - Lv, Ying-Ran AU - Ju, Chun-Mei 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 21
SP  - 2806
EP  - 2813
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2806.2813
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2806.2813
KW  - China
KW  -shRNA
KW  -RNA interference
KW  -Foot-and-mouth disease virus
KW  -L and 3D non-structural protein coding region
KW  -live stock
AB  - Foot-and-Mouth Disease Virus (FMDV) is a highly contagious vesicular disease affecting &gt;33 species of cloven-hoofed animals. Because of the limited effectiveness of existing antiviral approaches including vaccines and antiviral drugs, new strategies for controlling FMD outbreak are needed. RNA interference (RNAi) is considered to be one of the powerful emerging approaches to inhibit virus replication in mammalian cells. In this study, two short-hairpin RNA (shRNAs) expressing vectors (pShuttle-shRNA-L and pShuttle-shRNA-3D) targeting to the L and 3D non-structural protein coding regions of FMDV were employed to evaluate their antiviral ability in Baby Hamster Kidney cells (BHK-21). Results demonstrated that transfection of shRNA-expressing plasmids gave a significant weakened Cytopathic Effect (CPE) as well as a manifest decreased viral tilters and amount of FMDV mRNA copy numbers compared with the control cells post challenge of O/HK/2001. In this study, the data indicates that RNAi may be a potential therapeutic approach to limit the spread of FMDV in livestock.
ER  -